![]() ![]() The myeloma cells which contain HGPRT only can grow and replicate henceforth. The unfused myeloma cells can’t grow in the mixture because they lack HGPRT and thus cannot replicate. The resultant cell population is now cultured or cultivated in the HAT medium (the HAT medium is supplemented with hypoxanthine aminopterin thymidine). The resulting cell population is the fusion unit of hybrid cells, which is called a hybridoma. PEG brings the cells together and induces fusion. The cell mixture is shaken well for 3min. After the fusion of these two, the mixture is produced, which is treated further with PEG (polyethylene glycol). The next step is the fusion of HGPRT cells and B-lymphocytes. The myeloma cells are taken into account for the production of monoclonal antibodies. The cell contains a genetic marker (HGPRT –hypo xanthine phosphor ribosyl transferase), which permits an early solution of the resulting hybrid cells.These cells do not produce antibodies themselves.The myeloma cells are selected for only two features which are as follows: It is capable of multiplying indefinitely. The myeloma cells are fast-growing large cells of a hemopoietic portion of bone marrow. The steps to produce the monoclonal antibodies are as followsĮnsuring Safe and Effective Medications: An Overview of Pharmaceutical Manufacturing Processes and Quality Control Measures The fusing agent can be a defective virus (Sendai virus that characteristically causes cell fusion) or chemicals like polyethylene glycol. And this hybridoma possesses the immortal growth properties of the myeloma cell and the antibody-secreting property of the B-cell.The hybrid cell, which is produced as the result of this fusion, is called a hybridoma.In this method, the monoclonal antibodies are produced by fusing usually activated antibody-producing B-cells with myeloma cells.In the Vivo method (Using living animals like Mice).In Vitro Methods (Using cell lines in glassware).Monoclonal antibody production can be done by two methods. This quickly became one of the immunology’s key technologies, and they were awarded the Nobel Prize for the same in medicine and physiology in 1984. In 1975, two scientists, Georges Kohler and Cesar Milstein, devised a revolutionary method for preparing monoclonal antibodies. All molecules of a monoclonal antibody will have the same specificity. Therefore, such antibodies are known as monoclonal antibodies. This clone produces antibodies of a single specificity. The clone derived from the fusion of a single differentiated B-cells(antibody-forming) is a clone of a single B-cell. ![]() bioreactors or large flasks).These hybrid cells express the properties of both parental cell lines. ![]()
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